A1 Refereed original research article in a scientific journal

Assembly of split aptamers by dynamic pH-responsive covalent ligation



Authors: Aho Aapo, Virta Pasi

Publisher: ROYAL SOC CHEMISTRY

Publication year: 2023

Journal: Chemical Communications

Journal name in source: CHEMICAL COMMUNICATIONS

Journal acronym: CHEM COMMUN

Volume: 59

Issue: 38

First page : 5689

Last page: 5692

Number of pages: 4

ISSN: 1359-7345

DOI: https://doi.org/10.1039/d3cc01158e

Web address : https://doi.org/10.1039/D3CC01158E

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/179466137


Abstract
Reversible pH-responsive N-methoxyoxazolidine formation is used to ligate split aptamer fragments. Two twice-split models and one thrice-split model of CBA (cocaine-binding aptamer) were examined. The aptamer assembly was dynamic, proportional to the substrate concentration and occurred without interfering background ligation.

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Last updated on 2024-26-11 at 13:26