A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies
Tekijät: Knuutila Aapo, Rautanen Carita, Barkoff Alex-Mikael, Mertsola Jussi, He Qiushui
Kustantaja: ELSEVIER
Julkaisuvuosi: 2022
Journal: Journal of Immunological Methods
Tietokannassa oleva lehden nimi: JOURNAL OF IMMUNOLOGICAL METHODS
Lehden akronyymi: J IMMUNOL METHODS
Artikkelin numero: 113361
Vuosikerta: 510
Sivujen määrä: 4
ISSN: 0022-1759
eISSN: 1872-7905
DOI: https://doi.org/10.1016/j.jim.2022.113361
Verkko-osoite: https://doi.org/10.1016/j.jim.2022.113361
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/176744795
Current serological diagnosis of pertussis is usually done by ELISA to determine serum specific anti-pertussis toxin (PT) IgG antibodies. However, the ELISAs are often central-laboratory based, require trained staff, and have long turnaround times. A rapid point-of-care (POC) assay for pertussis serology would aid in both diagnosis and surveillance of the disease. In this study, a quantitative lateral flow assay (LFA) with fluorescent Eu-nanoparticle reporters was used for the detection of anti-PT antibodies from whole blood. The assay was eval-uated by testing overall 141 samples including 25 before and 116 one month after acellular pertussis booster vaccination. LFA results were compared to those obtained with standardized anti-PT IgG ELISAs with paired serum samples. Correlation between the assays was high (Pearson R = 0.832), and the achieved analytical sensitivity of the LFA was 29 IU/mL, which would be sufficient for clinically relevant cutoffs for determining recent infections. The paired samples, collected pre-and post-booster, demonstrated a significant increase in anti-PT IgG antibodies similar to that detected by ELISA. The developed LFA opens up several alternatives for a suitable POC test also in middle-and low-income countries.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
