A1 Refereed original research article in a scientific journal
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies
Authors: Knuutila Aapo, Rautanen Carita, Barkoff Alex-Mikael, Mertsola Jussi, He Qiushui
Publisher: ELSEVIER
Publication year: 2022
Journal: Journal of Immunological Methods
Journal name in source: JOURNAL OF IMMUNOLOGICAL METHODS
Journal acronym: J IMMUNOL METHODS
Article number: 113361
Volume: 510
Number of pages: 4
ISSN: 0022-1759
eISSN: 1872-7905
DOI: https://doi.org/10.1016/j.jim.2022.113361(external)
Web address : https://doi.org/10.1016/j.jim.2022.113361(external)
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/176744795(external)
Current serological diagnosis of pertussis is usually done by ELISA to determine serum specific anti-pertussis toxin (PT) IgG antibodies. However, the ELISAs are often central-laboratory based, require trained staff, and have long turnaround times. A rapid point-of-care (POC) assay for pertussis serology would aid in both diagnosis and surveillance of the disease. In this study, a quantitative lateral flow assay (LFA) with fluorescent Eu-nanoparticle reporters was used for the detection of anti-PT antibodies from whole blood. The assay was eval-uated by testing overall 141 samples including 25 before and 116 one month after acellular pertussis booster vaccination. LFA results were compared to those obtained with standardized anti-PT IgG ELISAs with paired serum samples. Correlation between the assays was high (Pearson R = 0.832), and the achieved analytical sensitivity of the LFA was 29 IU/mL, which would be sufficient for clinically relevant cutoffs for determining recent infections. The paired samples, collected pre-and post-booster, demonstrated a significant increase in anti-PT IgG antibodies similar to that detected by ELISA. The developed LFA opens up several alternatives for a suitable POC test also in middle-and low-income countries.
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