A1 Refereed original research article in a scientific journal

Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient




AuthorsLotila Johanna, Hyvärinen Tarja, Skottman Heli, Airas Laura, Narkilahti Susanna, Hagman Sanna

PublisherElsevier B.V.

Publication year2022

JournalStem Cell Research

Journal name in sourceStem Cell Research

Volume63

DOIhttps://doi.org/10.1016/j.scr.2022.102865

Web address https://doi.org/10.1016/j.scr.2022.102865

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/176045232


Abstract

Multiple sclerosis (MS) is a complex autoimmune disease of the central nervous system where the main pathogenetic events include demyelination and axonal degeneration. Here, we generated a human induced pluripotent stem cell (hiPSC) line from peripheral blood mononuclear cells of an MS patient utilizing Sendai virus reprogramming. The produced hiPSC line expressed pluripotency markers, differentiated into three germ layers, showed a normal karyotype and was free of virus vectors, transgenes and mycoplasma. Established hiPSCs are a valuable source for studies of MS disease modeling and drug discovery.


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