A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Impact of steric constraints on the product distribution of phosphate-branched oligonucleotide models of the large ribozymes
Tekijät: Lonnberg T, Kero KM
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2012
Journal: Organic and Biomolecular Chemistry
Tietokannassa oleva lehden nimi: ORGANIC & BIOMOLECULAR CHEMISTRY
Lehden akronyymi: ORG BIOMOL CHEM
Numero sarjassa: 3
Vuosikerta: 10
Numero: 3
Aloitussivu: 569
Lopetussivu: 574
Sivujen määrä: 6
ISSN: 1477-0520
DOI: https://doi.org/10.1039/c1ob06399e
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/1365142
To assess the extent to which steric constraints may influence the product distribution of the reactions of the large ribozymes, phosphate-branched oligonucleotides of varying length and sequence have been synthesized and their alkaline hydrolysis studied over a wide temperature range. At low temperatures, the branching trinucleoside-3 ',3 ',5 '-monophosphate moiety is hydrolyzed almost exclusively by P-O3 ' fission. At higher temperatures, P-O5 ' fission competes, accounting at most for 22% of the overall reaction. The results suggest that steric constraints imposed by the secondary structure of the reaction site may significantly contribute to the observed regioselectivity of the transesterification reactions catalyzed by the large ribozymes.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
