A1 Refereed original research article in a scientific journal
Impact of steric constraints on the product distribution of phosphate-branched oligonucleotide models of the large ribozymes
Authors: Lonnberg T, Kero KM
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2012
Journal: Organic and Biomolecular Chemistry
Journal name in source: ORGANIC & BIOMOLECULAR CHEMISTRY
Journal acronym: ORG BIOMOL CHEM
Number in series: 3
Volume: 10
Issue: 3
First page : 569
Last page: 574
Number of pages: 6
ISSN: 1477-0520
DOI: https://doi.org/10.1039/c1ob06399e
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/1365142
To assess the extent to which steric constraints may influence the product distribution of the reactions of the large ribozymes, phosphate-branched oligonucleotides of varying length and sequence have been synthesized and their alkaline hydrolysis studied over a wide temperature range. At low temperatures, the branching trinucleoside-3 ',3 ',5 '-monophosphate moiety is hydrolyzed almost exclusively by P-O3 ' fission. At higher temperatures, P-O5 ' fission competes, accounting at most for 22% of the overall reaction. The results suggest that steric constraints imposed by the secondary structure of the reaction site may significantly contribute to the observed regioselectivity of the transesterification reactions catalyzed by the large ribozymes.
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