Vilja Siitonen
PhD (FT)
vksiit@utu.fi Tykistökatu 6 Turku Office: 6108 ORCID identifier: https://orcid.org/0000-0002-1444-2622 |
Molecular biology; Protein biology; Bioactive small metabolites; Microbiology; Biotransformation, HPLC, MS, NMR, GC
I am working as a postdoctoral researcher at photomicrobes research group led by Prof. Yagut Allahverdiyeva-Rinne (www.utu.fi/photo-microbes). I study biotransformations and production of compounds by Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803. During my first postdoc I was studying secondary metabolites, purified and elucidated their structures by NMR. I finished my PhD in 2016 in which my focus was the biosynthesis of an anthracycline antibiotic Nogalamycin. Before starting my PhD I was studying fat production of Streptomyces and my Master thesis was about that. Before graduating as a M.Sc 2010, I spent an exchange year 2005-2006 in Austria, Salzburg.
I study biotransformations and production of compounds by Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803. In have also studied secondary metabolism of Streptomyces and related micro-organisms. I studied the genes and proteins in vitro and in vivo as well as the compounds produced by the strains or reactions and solved structures of bioactive compounds by using MS and NMR.
I taught hydrogen production, measuring and biotransformation as a part of a photosynthetis course (2022).
I taught a biotransformation course as a part of a photosynthetic laboratory course (2022).
I taught a HPLC laboratory course as a part of a photosynthetic laboratory course (2021).
I taught a course on molecular biology using Streptomyces (2011).
In addition to that, I am supervising one master thesis at the moment, I have supervised 2 Master thesis works (experimental section in one and the whole work in the other), 3 Bachelor of Science works and planned and supervised 12 project workers works in the laboratory (1–6 months each).
- Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli (2022)
- Biotechnology for Biofuels and Bioproducts
(Refereed journal article or data article (A1)) - Single cell mutant selection for metabolic engineering of actinomycetes (2022)
- Metabolic Engineering
(Refereed journal article or data article (A1)) - Biosynthesis of Diverse Type II Polyketide Core Structures in Streptomyces coelicolor M1152 (2021)
- ACS Synthetic Biology
(Refereed journal article or data article (A1)) - Differential regulation of undecylprodigiosin biosynthesis in the yeast-scavenging Streptomyces strain MBK6 (2021)
- FEMS Microbiology Letters
(Refereed journal article or data article (A1)) - Evolution‐Guided Engineering of Non‐Heme Iron Enzymes Involved in Nogalamycin Biosynthesis (2020)
- FEBS Journal
(Refereed journal article or data article (A1)) - Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated Halophilic Streptomonospora sp. PA3 (2020)
- Frontiers in Microbiology
(Refereed journal article or data article (A1)) - Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis (2020)
- Acta Crystallographica Section F: Structural Biology Communications
(Refereed journal article or data article (A1)) - The Rieske Oxygenase SnoT Catalyzes 2''-Hydroxylation of L-Rhodosamine in Nogalamycin Biosynthesis (2020)
- ChemBioChem
(Refereed journal article or data article (A1)) - Enzymatic Synthesis of the C-glycosidic Moiety of Nogalamycin R (2018)
- ACS Chemical Biology
(Refereed journal article or data article (A1)) - Discovery of the Showdomycin Gene Cluster from Streptomyces showdoensis ATCC 15227 Yields Insight into the Biosynthetic Logic of C-Nucleoside Antibiotics (2017)
- ACS Chemical Biology
(Refereed journal article or data article (A1)) - Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway (2016)
- Proceedings of the National Academy of Sciences of the United States of America
(Refereed journal article or data article (A1)) - Glycosylation and Glycodiversification in Polyketide Antibiotics: Unraveling Biosynthetic Steps in Nogalamycin Formation (2016) Siitonen Vilja
(Doctoral dissertation (article) (G5)) - Laboratory course on Streptomyces genetics and secondary metabolism (2016)
- Biochemistry and Molecular Biology Education
(Refereed journal article or data article (A1)) - Crystal structure of the glycosyltransferase SnogD from the biosynthetic pathway of nogalamycin in Streptomyces nogalater (2012)
- FEBS Journal
(Refereed journal article or data article (A1)) - Discovery of a Two-Component Monooxygenase SnoaW/SnoaL2 Involved in Nogalamycin Biosynthesis (2012)
- Chemistry and Biology
(Refereed journal article or data article (A1)) - Identification of Late-Stage Glycosylation Steps in the Biosynthetic Pathway of the Anthracycline Nogalamycin (2012)
- ChemBioChem
(Refereed journal article or data article (A1))