Tuomas Huovinen
tuohuo@utu.fi +358 29 450 4588 +358 50 476 5558 Kiinamyllynkatu 10 Turku |
Mutagenesis, recombinant antibodies, phage display, directed evolution
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- Advancing recombinant antibody production in E. coli: Optimization of expression and purification via dual GFP promoter and imaging technology (2025)
- Protein Expression and Purification
- Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassays (2024)
- Scientific Reports
- Epitope-specific antibody fragments block aggregation of AGelD187N, an aberrant peptide in gelsolin amyloidosis (2024)
- Journal of Biological Chemistry
- Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects (2023)
- Scientific Reports
- Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins (2022)
- ACS Synthetic Biology
- Combinatorial mutagenesis with alternative CDR-L1 and-H2 loop lengths contributes to affinity maturation of antibodies (2021)
- New Biotechnology
- Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays (2021)
- Analytical Chemistry
- Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR (2020)
- ACS Omega
- Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins (2020)
- Nucleic Acids Research
- Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries (2019)
- Microbial Cell Factories
- Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E-coli (2017)
- Scientific Reports
- Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library – Phage display derived HDL antibodies (2016)
- Clinical Biochemistry
- Fast conversion of scFv to Fab antibodies using type IIs restriction enzymes (2015)
- Journal of Immunological Methods
- Lactobacillus helveticus MIMLh5-specific antibodies for detection of S-layer protein in Grana Padano protected-designation-of-origin cheese (2014)
- Applied and Environmental Microbiology
- The selection performance of an antibody library displayed on filamentous phage coat proteins p9, p3 and truncated p3 (2014)
- BMC Research Notes
- A simple heterogeneous one-step assay for screening estrogenic compounds (2013)
- Biotechnology Letters
- Two ScFv antibody libraries derived from identical VLVH framework with different binding site designs display distinct binding profiles (2013)
- Protein Engineering, Design and Selection
- Determination of Dimethachlon in Tobacco by Time-resolved Fluorescence Immunoassay (2012)
- Chinese Journal of Analytical Chemistry
- Homogenous M13 bacteriophage quantification assay using switchable lanthanide fluorescence probes (2012)
- Biotechniques
- Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification (2012)
- PLoS ONE
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