Tuomas Huovinen
tuohuo@utu.fi +358 29 450 4588 +358 50 476 5558 Kiinamyllynkatu 10 Turku ORCID identifier: https://orcid.org/0000-0003-0960-3249 |
Research
Mutagenesis, recombinant antibodies, phage display, directed evolution
Mutagenesis, recombinant antibodies, phage display, directed evolution
Publications 
1 of 2
- Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassays (2024)
- Scientific Reports
(A1 Refereed original research article in a scientific journal) - Epitope-specific antibody fragments block aggregation of AGelD187N, an aberrant peptide in gelsolin amyloidosis (2024)
- Journal of Biological Chemistry
(A1 Refereed original research article in a scientific journal) - Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects (2023)
- Scientific Reports
(A1 Refereed original research article in a scientific journal) - Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins (2022)
- ACS Synthetic Biology
(A1 Refereed original research article in a scientific journal) - Combinatorial mutagenesis with alternative CDR-L1 and-H2 loop lengths contributes to affinity maturation of antibodies (2021)
- New Biotechnology
(A1 Refereed original research article in a scientific journal) - Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays (2021)
- Analytical Chemistry
(A1 Refereed original research article in a scientific journal) - Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR (2020)
- ACS Omega
(A1 Refereed original research article in a scientific journal) - Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins (2020)
- Nucleic Acids Research
(A1 Refereed original research article in a scientific journal) - Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries (2019)
- Microbial Cell Factories
(A1 Refereed original research article in a scientific journal) - Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E-coli (2017)
- Scientific Reports
(A1 Refereed original research article in a scientific journal) - Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library – Phage display derived HDL antibodies (2016)
- Clinical Biochemistry
(A1 Refereed original research article in a scientific journal) - Fast conversion of scFv to Fab antibodies using type IIs restriction enzymes (2015)
- Journal of Immunological Methods
(A1 Refereed original research article in a scientific journal) - Lactobacillus helveticus MIMLh5-specific antibodies for detection of S-layer protein in Grana Padano protected-designation-of-origin cheese (2014)
- Applied and Environmental Microbiology
(A1 Refereed original research article in a scientific journal) - The selection performance of an antibody library displayed on filamentous phage coat proteins p9, p3 and truncated p3 (2014)
- BMC Research Notes
(A1 Refereed original research article in a scientific journal) - A simple heterogeneous one-step assay for screening estrogenic compounds (2013)
- Biotechnology Letters
(A1 Refereed original research article in a scientific journal) - Two ScFv antibody libraries derived from identical VLVH framework with different binding site designs display distinct binding profiles (2013)
- Protein Engineering, Design and Selection
(A1 Refereed original research article in a scientific journal) - Determination of Dimethachlon in Tobacco by Time-resolved Fluorescence Immunoassay (2012)
- Chinese Journal of Analytical Chemistry
(A1 Refereed original research article in a scientific journal) - Homogenous M13 bacteriophage quantification assay using switchable lanthanide fluorescence probes (2012)
- Biotechniques
(A1 Refereed original research article in a scientific journal) - Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification (2012)
- PLoS ONE
(A1 Refereed original research article in a scientific journal) - Enhanced error-prone RCA mutagenesis by concatemer resolution (2011)
- Plasmid
(A1 Refereed original research article in a scientific journal)
Turun yliopiston Tutkimustietojärjestelmän portaali