Tuomas Huovinen
tuohuo@utu.fi +358 29 450 4588 +358 50 476 5558 Kiinamyllynkatu 10 Turku ORCID identifier: https://orcid.org/0000-0003-0960-3249 |
Research
Mutagenesis, recombinant antibodies, phage display, directed evolution
Mutagenesis, recombinant antibodies, phage display, directed evolution
Publications 
1 of 2
- Advancing recombinant antibody production in E. coli: Optimization of expression and purification via dual GFP promoter and imaging technologySite-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR (2025)
- Protein Expression and PurificationMicrobial Cell Factories
(A1 Refereed original research article in a scientific journal) - Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassaysSplit & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins (2024)
- Scientific ReportsNucleic Acids Research
(A1 Refereed original research article in a scientific journal) - Epitope-specific antibody fragments block aggregation of AGelD187N, an aberrant peptide in gelsolin amyloidosisImprovement of Fab expression by screening combinatorial synonymous signal sequence libraries (2024)
- Journal of Biological Chemistry
(A1 Refereed original research article in a scientific journal) - Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects (2023)
- Scientific ReportsJournal of Immunological Methods
(A1 Refereed original research article in a scientific journal) - Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPinsIdentification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library – Phage display derived HDL antibodiesLactobacillus helveticus MIMLh5-specific antibodies for detection of S-layer protein in Grana Padano protected-designation-of-origin cheese (2022)
- ACS Synthetic BiologyClinical Biochemistry
(A1 Refereed original research article in a scientific journal) - Combinatorial mutagenesis with alternative CDR-L1 and-H2 loop lengths contributes to affinity maturation of antibodies (2021)
- New Biotechnology
(A1 Refereed original research article in a scientific journal) - Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays (2021)
- Analytical ChemistryApplied and Environmental MicrobiologyBiotechnology Letters
(A1 Refereed original research article in a scientific journal) - Two ScFv antibody libraries derived from identical VLVH framework with different binding site designs display distinct binding profiles (2020)
- ACS OmegaBMC Research Notes
(A1 Refereed original research article in a scientific journal) - (2020)
(A1 Refereed original research article in a scientific journal) - Homogenous M13 bacteriophage quantification assay using switchable lanthanide fluorescence probes (2019)
(A1 Refereed original research article in a scientific journal) - Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E-coli (2017)
- Scientific Reports
(A1 Refereed original research article in a scientific journal) - 2016
(A1 Refereed original research article in a scientific journal) - Fast conversion of scFv to Fab antibodies using type IIs restriction enzymes (2015)
(A1 Refereed original research article in a scientific journal) - (2014)
(A1 Refereed original research article in a scientific journal) - The selection performance of an antibody library displayed on filamentous phage coat proteins p9, p3 and truncated p3 (2014)
(A1 Refereed original research article in a scientific journal) - A simple heterogeneous one-step assay for screening estrogenic compounds (2013)
(A1 Refereed original research article in a scientific journal) - (2013)
- Protein Engineering, Design and Selection
(A1 Refereed original research article in a scientific journal) - Determination of Dimethachlon in Tobacco by Time-resolved Fluorescence Immunoassay (2012)
- Chinese Journal of Analytical Chemistry
(A1 Refereed original research article in a scientific journal) - (2012)
- Biotechniques
(A1 Refereed original research article in a scientific journal) - Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification (2012)
- PLoS ONE
(A1 Refereed original research article in a scientific journal)
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