A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A peptide from ICAM-2 binds to the leukocyte integrin CD11a/CD18 and inhibits endothelial cell adhesion
Tekijät: Li R.; Nortamo P.; Valmu L.; Tolvanen M.; Huuskonen J.; Kantor C.; Gahmberg C.G.
Kustantaja: Elsevier BV
Julkaisuvuosi: 1993
Journal: Journal of Biological Chemistry
Tietokannassa oleva lehden nimi: Journal of Biological Chemistry
Lehden akronyymi: J Biol Chem
Vuosikerta: 268
Numero: 23
Aloitussivu: 17513
Lopetussivu: 17518
ISSN: 0021-9258
DOI: https://doi.org/10.1016/S0021-9258(19)85363-2
Verkko-osoite: https://doi.org/10.1016/s0021-9258(19)85363-2
Tiivistelmä
Numerous leukocyte functions depend on adhesive intercellular interactions. The leukocyte-specific integrins CD11a/CD18 (lymphocyte function-associated antigen-1 (LFA-1)) and CD11b/CD18 (complement type 3 receptor (Mac-1)), which bind to the intercellular adhesion molecules ICAM-1 and ICAM-2, play a key role in adhesion. Little is known about the binding in molecular detail. We have now defined a peptide region from the first immunoglobulin domain of ICAM-2 that is specifically involved in binding to CD11a/CD18. A synthetic peptide from this part of ICAM-2, covering residues 21-42, bound to purified CD11a/CD18 and inhibited the adhesion of endothelial cells to this integrin. It also inhibited the binding of B lymphoblastoid cells to endothelial cells. Leukocytes bound to the peptide coated on plastic. Several shorter peptides from the same region showed less or no activity.
Numerous leukocyte functions depend on adhesive intercellular interactions. The leukocyte-specific integrins CD11a/CD18 (lymphocyte function-associated antigen-1 (LFA-1)) and CD11b/CD18 (complement type 3 receptor (Mac-1)), which bind to the intercellular adhesion molecules ICAM-1 and ICAM-2, play a key role in adhesion. Little is known about the binding in molecular detail. We have now defined a peptide region from the first immunoglobulin domain of ICAM-2 that is specifically involved in binding to CD11a/CD18. A synthetic peptide from this part of ICAM-2, covering residues 21-42, bound to purified CD11a/CD18 and inhibited the adhesion of endothelial cells to this integrin. It also inhibited the binding of B lymphoblastoid cells to endothelial cells. Leukocytes bound to the peptide coated on plastic. Several shorter peptides from the same region showed less or no activity.
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