B1 Vertaisarvioimaton kirjoitus tieteellisessä lehdessä
Guidelines for reporting methods to estimate metabolic rates by aquatic intermittent-flow respirometry
Tekijät: Killen Shaun S., Christensen Emil AF, Cortese Daphne, Závorka Libor, Norin Tommy, Cotgrove Lucy, Crespel Amélie, Munson Amelia, Nati Julie JH, Papatheodoulou Magdalene, McKenzie David J
Kustantaja: COMPANY BIOLOGISTS LTD
Julkaisuvuosi: 2021
Journal: Journal of Experimental Biology
Tietokannassa oleva lehden nimi: JOURNAL OF EXPERIMENTAL BIOLOGY
Lehden akronyymi: J EXP BIOL
Artikkelin numero: ARTN jeb242522
Vuosikerta: 224
Numero: 18
Sivujen määrä: 13
ISSN: 0022-0949
DOI: https://doi.org/10.1242/jeb.242522
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/67547037
Interest in the measurement of metabolic rates is growing rapidly, because of the importance of metabolism in advancing our understanding of organismal physiology, behaviour, evolution and responses to environmental change. The study of metabolism in aquatic animals is undergoing an especially pronounced expansion, with more researchers utilising intermittent-flow respirometry as a research tool than ever before. Aquatic respirometry measures the rate of oxygen uptake as a proxy for metabolic rate, and the intermittent-flow technique has numerous strengths for use with aquatic animals, allowing metabolic rate to be repeatedly estimated on individual animals over several hours or days and during exposure to various conditions or stimuli. There are, however, no published guidelines for the reporting of methodological details when using this method. Here, we provide the first guidelines for reporting intermittent-flow respirometry methods, in the form of a checklist of criteria that we consider to be the minimum required for the interpretation, evaluation and replication of experiments using intermittent-flow respirometry. Furthermore, using a survey of the existing literature, we show that there has been incomplete and inconsistent reporting of methods for intermittent-flow respirometry over the past few decades. Use of the provided checklist of required criteria by researchers when publishing their work should increase consistency of the reporting of methods for studies that use intermittent-flow respirometry. With the steep increase in studies using intermittent-flow respirometry, now is the ideal time to standardise reporting of methods, so that - in the future - data can be properly assessed by other scientists and conservationists.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
