Background: Scarce data exist on the impact of granulocyte-colony stimulating factor (G-CSF) type on the mobilizing capacity of CD34⁺ cells, graft cellular composition, and outcome in myeloma (MM) patients.

Patients and methods: In this prospective multicenter study, 70 patients with MM received filgrastim (FIL) and 20 patients received pegfilgrastim (PEG) as a G-CSF after low-dose cyclophosphamide. Flow cytometry was used to analyze the mobilization of CD34⁺ cells and cellular composition of blood grafts, hematologic recovery, and survival after auto-SCT according to the G-CSF choice.

Results: The CD34⁺ cell yield of the first apheresis was higher in the FIL group (5.3 vs. 4.2 × 10⁶ /kg, p = .025). The better mobilizing capacity was observed in the FIL group especially after bortezomib-based induction based on the higher first apheresis yield of CD34⁺ cells (7.5 vs. 4.4 × 10⁶ /kg, p = .001). The median CD19⁺ cell count (1.0 vs. 0.4 × 10⁶ /kg, p = .010) and the number of CD3⁺ T lymphocytes (43.1 vs. 31.8 × 10⁶ /kg, p = .122) in the infused graft were higher in the patients mobilized with FIL. Both early (day +15) (56 vs. 108 × 10⁹ /L, p = .002) and later platelet recovery at 6 months (191 vs. 226 × 10⁹ /L, p = .026) were faster in the PEG group.

Conclusion: G-CSF type seems to impact on the mobilization capacity and cellular composition of infused graft and also platelet recovery post-transplant. A randomized study might be warranted to verify the effects of G-CSF choice in the mobilization field.