A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Perfluoroalkyl substances are increased in patients with late-onset ulcerative colitis and induce intestinal barrier defects ex vivo in murine intestinal tissue




Julkaisun tekijät: Fart Frida, Salihović Samira, McGlinchey Aidan, Gareau Melanie G, Orešič Matej, Halfvarson Jonas, Hyötyläinen Tuulia, Schoultz Ida

Kustantaja: TAYLOR & FRANCIS LTD

Julkaisuvuosi: 2021

Journal: Scandinavian Journal of Gastroenterology

Tietokannassa oleva lehden nimi: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY

Lehden akronyymi: SCAND J GASTROENTERO

Sivujen määrä: 10

ISSN: 0036-5521

eISSN: 1502-7708

DOI: http://dx.doi.org/10.1080/00365521.2021.1961306


Tiivistelmä

Background

Environmental factors are strongly implicated in late-onset of inflammatory bowel disease. Here, we investigate whether high levels of perfluoroalkyl substances are associated with (1) late-onset inflammatory bowel disease, and (2) disturbances of the bile acid pool. We further explore the effect of the specific perfluoroalkyl substance perfluorooctanoic acid on intestinal barrier function in murine tissue.

Methods

Serum levels of perfluoroalkyl substances and bile acids were assessed by ultra-performance liquid chromatography coupled to a triple-quadrupole mass spectrometer in matched samples from patients with ulcerative colitis (n = 20) and Crohn's disease (n = 20) diagnosed at the age of >= 55 years. Age and sex-matched blood donors (n = 20), were used as healthy controls. Ex vivo Ussing chamber experiments were performed to assess the effect of perfluorooctanoic acid on ileal and colonic murine tissue (n = 9).

Results

The total amount of perfluoroalkyl substances was significantly increased in patients with ulcerative colitis compared to healthy controls and patients with Crohn's disease (p < .05). Ex vivo exposure to perfluorooctanoic acid induced a significantly altered ileal and colonic barrier function. The distribution of bile acids, as well as the correlation pattern between (1) perfluoroalkyl substances and (2) bile acids, differed between patient and control groups.

Discussion

Our results demonstrate that perfluoroalkyl substances levels are increased in patients with late-onset ulcerative colitis and may contribute to the disease by inducing a dysfunctional intestinal barrier.


Ladattava julkaisu

This is an electronic reprint of the original article.
This reprint may differ from the original in pagination and typographic detail. Please cite the original version.




Last updated on 2021-22-09 at 14:11