A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Talin rod domain-containing protein 1 (TLNRD1) is a novel actin-bundling protein which promotes filopodia formation
Tekijät: Cowell Alana R, Jacquemet Guillaume, Singh Abhimanyu K, Varela Lorena, Nylund Anna S, Ammon York-Christoph, Brown David G, Akhmanova Anna, Ivaska Johanna, Goult Benjamin T
Kustantaja: Rockefeller University Press
Julkaisuvuosi: 2021
Journal: Journal of Cell Biology
Tietokannassa oleva lehden nimi: The Journal of cell biology
Lehden akronyymi: J Cell Biol
Artikkelin numero: e202005214
Vuosikerta: 220
Numero: 9
ISSN: 0021-9525
eISSN: 1540-8140
DOI: https://doi.org/10.1083/jcb.202005214
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/66601798
Talin is a mechanosensitive adapter protein that couples integrins to the cytoskeleton. Talin rod domain-containing protein 1 (TLNRD1) shares 22% homology with the talin R7R8 rod domains, and is highly conserved throughout vertebrate evolution, although little is known about its function. Here we show that TLNRD1 is an α-helical protein structurally homologous to talin R7R8. Like talin R7R8, TLNRD1 binds F-actin, but because it forms a novel antiparallel dimer, it also bundles F-actin. In addition, it binds the same LD motif-containing proteins, RIAM and KANK, as talin R7R8. In cells, TLNRD1 localizes to actin bundles as well as to filopodia. Increasing TLNRD1 expression enhances filopodia formation and cell migration on 2D substrates, while TLNRD1 down-regulation has the opposite effect. Together, our results suggest that TLNRD1 has retained the diverse interactions of talin R7R8, but has developed distinct functionality as an actin-bundling protein that promotes filopodia assembly.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
