A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Maculosin, a non-toxic antioxidant compound isolated from Streptomyces sp. KTM18
Tekijät: Paudel Babita, Maharjan Rukusha Rajbhandari Prajwal, Aryal Niraj, Aziz Saefuddin, Bhattarai Keshab, Baral Bikash, Malla Rajani, Bhattarai Hari Datta
Kustantaja: TAYLOR & FRANCIS LTD
Julkaisuvuosi: 2021
Journal: Pharmaceutical Biology
Tietokannassa oleva lehden nimi: PHARMACEUTICAL BIOLOGY
Lehden akronyymi: PHARM BIOL
Vuosikerta: 59
Numero: 1
Aloitussivu: 933
Lopetussivu: 936
Sivujen määrä: 4
ISSN: 1388-0209
eISSN: 1744-5116
DOI: https://doi.org/10.1080/13880209.2021.1946091
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/66590907
Context Streptomyces species are prolific sources of bioactive secondary metabolites known especially for their antimicrobial and anticancer activities. Objective This study sought to isolate and characterize antioxidant molecules biosynthesized by Streptomyces sp. KTM18. The antioxidant potential of an isolated compound and its toxicity were accessed. Materials and methods The compound was purified using bioassay-guided chromatography techniques. Nuclear magnetic resonance (NMR) experiments were carried out for structure elucidation. The antioxidant potential of the isolated compound was determined using DPPH free radical scavenging assay. The toxicity of the isolated compound was measured using a brine shrimp lethality (BSL) assay. Results Ethyl acetate extract of Streptomyces sp. KTM18 showed more than 90% inhibition of DPPH free radical at 50 mu g/mL of the test concentration. These data were the strongest among 13 Streptomyces isolates (KTM12-KTM24). The active molecule was isolated and characterized as maculosin (molecular formula, C14H16N2O3 as determined by the [M + H](+) peak at 261.1259). The DPPH free radical scavenging activity of pure maculosin was higher (IC50, 2.16 +/- 0.05 mu g/mL) than that of commercial butylated hydroxyanisole (BHA) (IC50, 4.8 +/- 0.05 mu g/mL). No toxicity was observed for maculosin (LD50, <128 mu g/mL) in brine shrimp lethality assay (BSLA) up to the compound's antioxidant activity (IC50) concentration range. The commercial standard, berberine chloride, showed toxicity in BSLA with an LD50 value of 8.63 +/- 0.15 mu g/mL. Conclusions Maculosin may be a leading drug candidate in various cosmetic and therapeutic applications owing to its strong antioxidant and non-toxic properties.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
