A1 Refereed original research article in a scientific journal
A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics
Authors: Jalkanen Pinja, Pasternack Arja, Maljanen Sari, Melén Krister, Kolehmainen Pekka, Huttunen Moona, Lundberg Rickard, Tripathi Lav, Khan Hira, Ritvos Mikael A, Naves Rauno, Haveri Anu, Österlund Pamela, Kuivanen Suvi, Jääskeläinen Anne J, Kurkela Satu, Lappalainen Maija, Rantasärkkä Kaisa, Vuorinen Tytti, Hytönen Jukka, Waris Matti, Tauriainen Sisko, Ritvos Olli, Kakkola Laura, Julkunen Ilkka
Publisher: Oxford Academic
Publication year: 2021
Journal: Journal of Infectious Diseases
Journal name in source: The Journal of infectious diseases
Journal acronym: J Infect Dis
Volume: 224
Issue: 2
First page : 218
Last page: 228
ISSN: 0022-1899
eISSN: 1537-6613
DOI: https://doi.org/10.1093/infdis/jiab222
Web address : https://academic.oup.com/jid/article/224/2/218/6255671
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/66577579
Background: Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates.
Methods: We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), S1 and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results.
Results: The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test results.
Conclusions: The data indicate a combination of SARS-CoV-2 S1 or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Downloadable publication This is an electronic reprint of the original article. |  |
