A1 Refereed original research article in a scientific journal
Preparation of ⁶⁸Ga-labelled DOTA-peptides using a manual labelling approach for small-animal PET imaging
Authors: Romero E, Martínez A, Oteo M, García A, Morcillo MA
Publisher: Elsevier
Publication year: 2016
Journal acronym: Appl Radiat Isot
Volume: 107
First page : 113
Last page: 120
DOI: https://doi.org/10.1016/j.apradiso.2015.10.005
Web address : https://www.sciencedirect.com/science/article/pii/S0969804315302037?via%3Dihub
(68)Ga-DOTA-peptides are a promising PET radiotracers used in the detection of different tumours types due to their ability for binding specifically receptors overexpressed in these. Furthermore, (68)Ga can be produced by a (68)Ge/(68)Ga generator on site which is a very good alternative to cyclotron-based PET isotopes. Here, we describe a manual labelling approach for the synthesis of (68)Ga-labelled DOTA-peptides based on concentration and purification of the commercial (68)Ga/(68)Ga generator eluate using an anion exchange-cartridge. (68)Ga-DOTA-TATE was used to image a pheochromocytoma xenograft mouse model by a microPET/CT scanner. The method described provides satisfactory results, allowing the subsequent (68)Ga use to label DOTA-peptides. The simplicity of the method along with its implementation reduced cost, makes it useful in preclinical PET studies.
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