A1 Journal article – refereed

Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery

List of Authors: van der Kolk Birgitta W., Muniandy Maheswary, Kaminska Dorota, Alvarez Marcus, Ko Arthur, Miao Zong, Valsesia Armand, Langin Dominique, Vaittinen Maija, Pääkkönen Mirva, Jokinen Riikka, Kaye Sanna, Heinonen Sini, Virtanen Kirsi A., Andersson Daniel P., Männistö Ville, Saris Wim H., Astrup Arne, Rydén Mikael, Blaak Ellen E., Pajukanta Päivi, Pihlajamäki Jussi, Pietiläinen Kirsi H.

Publisher: Oxford University Press

Publication year: 2021

Journal: Journal of Clinical Endocrinology and Metabolism

Journal name in source: The Journal of clinical endocrinology and metabolism

Volume number: 106

Issue number: 5

ISSN: 1945-7197

eISSN: 1945-7197

DOI: http://dx.doi.org/10.1210/clinem/dgab072

URL: https://academic.oup.com/jcem/article/106/5/1312/6131733



Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.


To investigate how SAT mitochondria change following diet-induced and bariatric surgery–induced weight-loss interventions in 4 independent weight-loss studies.


The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n = 261) and 6-month weight-maintenance (n = 121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n = 172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in 2 studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n = 19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n = 49) and 5-year (n = 37) follow-up.


Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; ingenuity pathway analysis [IPA] z-scores: −8.7 following LCD, −4.4 following weight maintenance; CRYO: IPA z-score: −5.6, all P < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).


Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.

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Last updated on 2021-24-06 at 08:21