A1 Refereed original research article in a scientific journal

The pursuit of resin-dentin bond durability: Simultaneous enhancement of collagen structure and polymer network formation in hybrid layers




AuthorsStape Thiago Henrique Scarabello, Mutluay Mustafa Murat, Tjäderhane Leo, Uurasjärvi Emilia, Koistinen Arto, Tezvergil-Mutluay Arzu

PublisherElsevier Inc.

Publication year2021

JournalDental Materials

Journal name in sourceDental Materials

Volume37

Issue7

First page 1083

Last page1095

ISSN0109-5641

eISSN1879-0097

DOIhttps://doi.org/10.1016/j.dental.2021.03.010(external)

Web address https://doi.org/10.1016/j.dental.2021.03.010(external)

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/59150297(external)


Abstract

Objective
Imperfect polymer formation as well as collagen’s susceptibility to enzymatic degradation increase the vulnerability of hybrid layers over time. This study investigated the effect of new dimethyl sulfoxide (DMSO)-containing pretreatments on long-term bond strength, hybrid layer quality, monomer conversion and collagen structure.
Methods
H3PO4-etched mid-coronal dentin surfaces from extracted human molars (n = 8) were randomly treated with aqueous and ethanolic DMSO solutions or following the ethanol-wet bonding technique. Dentin bonding was performed with a three-step etch-and-rinse adhesive. Resin-dentin beams (0.8 mm2) were stored in artificial saliva at 37 °C for 24 h and 2.5 years, submitted to microtensile bond strength testing at 0.5 mm/min and semi-quantitative SEM nanoleakage analysis (n = 8). Micro-Raman spectroscopy was used to determine the degree of conversion at different depths in the hybrid layer (n = 6). Changes in the apparent modulus of elasticity of demineralized collagen beams measuring 0.5 × 1.7 × 7 mm (n = 10) and loss of dry mass (n = 10) after 30 days were calculated via three-point bending and precision weighing, respectively.
Results
DMSO-containing pretreatments produced higher bond strengths, which did not change significantly over time presenting lower incidence of water-filled zones. Higher uniformity in monomer conversion across the hybrid layer occurred for all pretreatments. DMSO-induced collagen stiffening was reversible in water, but with lower peptide solubilization.
Significance
Improved polymer formation and higher stability of the collagen-structure can be attributed to DMSO’s unique ability to simultaneously modify both biological and resin components within the hybrid layer. Pretreatments composed of DMSO/ethanol may be a viable-effective alternative to extend the longevity of resin-dentin bonds.


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