A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro
Tekijät: Saukko Annina EA, Nykänen Olli, Sarin Jaakko K, Nissi Mikko J, te Moller Nikae CR, Weinans Harrie, Mancini Irina AD, Visser Jetze, Brommer Harold, van Weeren P Réné, Malda Jos, Grinstaff Mark W, Töyras Juha
Kustantaja: WILEY
Julkaisuvuosi: 2022
Lehti: Journal of Orthopaedic Research
Tietokannassa oleva lehden nimi: JOURNAL OF ORTHOPAEDIC RESEARCH
Lehden akronyymi: J ORTHOP RES
Vuosikerta: 40
Numero: 3
Aloitussivu: 703
Lopetussivu: 711
Sivujen määrä: 9
ISSN: 0736-0266
eISSN: 1554-527X
DOI: https://doi.org/10.1002/jor.25066
Julkaisun avoimuus kirjaamishetkellä: Avoimesti saatavilla
Julkaisukanavan avoimuus : Osittain avoin julkaisukanava
Verkko-osoite: https://doi.org/10.1002/jor.25066
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/58642799
Rinnakkaistallenteen lisenssi: CC BY
Rinnakkaistallennetun julkaisun versio: Kustantajan versio
To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning. Posttraumatic changes include proteoglycan (PG) loss and elevated water content. Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes. Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture. Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies. Control samples were collected from three nonoperated ponies. The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points. As a reference, equilibrium moduli, dynamic moduli, and PG content were measured. Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min. Significant Spearman's rank correlations were obtained at 20 and 24 h (rho = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content. QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
