A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A Noninvasive Method for Quantifying Cerebral Metabolic Rate of Oxygen by Hybrid PET/MRI: Validation in a Porcine Model
Tekijät: Narciso Lucas, Ssali Tracy, Liu Linshan, Biernaski Heather, Butler John, Morrison Laura, Hadway Jennifer, Corsaut Jeffrey, Hicks Justin W, Langham Michael C, Wehrli Felix W, Iida Hidehiro, St Lawrence Keith
Kustantaja: Society of Nuclear Medicine
Julkaisuvuosi: 2021
Journal: Journal of Nuclear Medicine
Tietokannassa oleva lehden nimi: Journal of nuclear medicine : official publication, Society of Nuclear Medicine
Lehden akronyymi: J Nucl Med
Vuosikerta: 62
Numero: 12
Aloitussivu: 1789
Lopetussivu: 1796
ISSN: 0161-5505
eISSN: 1535-5667
DOI: https://doi.org/10.2967/jnumed.120.260521
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/53971765
The gold standard for imaging the cerebral metabolic rate of oxygen (CMRO2) is positron emission tomography (PET); however, it is an invasive and complex procedure that also requires correction for recirculating 15O-H2O and the blood-borne activity. We propose a noninvasive reference-based hybrid PET/magnetic resonance imaging (MRI) method that uses functional MRI techniques to calibrate 15O-O2-PET data. Here, PET/MR imaging of oxidative metabolism (PMROx) was validated in an animal model by comparison to PET-alone measurements. Additionally, we investigated if the MRI-perfusion technique arterial spin labelling (ASL) could be used to further simplify PMROx by replacing 15O-H2O-PET, and if the PMROx was sensitive to anesthetics-induced changes in metabolism. Methods: 15O-H2O and 15O-O2 PET data were acquired in a hybrid PET/MR scanner (3 T Siemens Biograph mMR), together with simultaneous functional MRI (OxFlow and ASL), from juvenile pigs (n = 9). Animals were anesthetized with 3% isoflurane and 6 mL/kg/h propofol for the validation experiments and arterial sampling was performed for PET-alone measurements. PMROx estimates were obtained using whole-brain (WB) CMRO2 from OxFlow and local cerebral blood flow (CBF) from either noninvasive 15O-H2O-PET or ASL (PMROxASL). Changes in metabolism were investigated by increasing the propofol infusion to 20 mL/kg/h. Results: Good agreement and correlation were observed between regional CMRO2 measurements from PMROx and PET-alone. No significant differences were found between OxFlow and PET-only measurements of WB oxygen extraction fraction (0.30 ± 0.09 and 0.31 ± 0.09) and CBF (54.1 ± 16.7 and 56.6 ± 21.0 mL/100 g/min), or between PMROx and PET-only CMRO2 estimates (1.89 ± 0.16 and 1.81 ± 0.10 mLO2/100 g/min). Moreover, PMROx and PMROxASL were sensitive to propofol-induced reduction in CMRO2 Conclusion: This study provides initial validation of a noninvasive PET/MRI technique that circumvents many of the complexities of PET CMRO2 imaging. PMROx does not require arterial sampling and has the potential to reduce PET imaging to 15O-O2 only; however, future validation involving human participants are required.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
