A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Phenolic Metabolites in the Urine and Plasma of Healthy Men After Acute Intake of Purple Potato Extract Rich in Methoxysubstituted Monoacylated Anthocyanins
Tekijät: Jokioja Johanna, Percival Jasmine, Philo Mark, Yang Baoru, Kroon Paul A., Linderborg Kaisa M.
Kustantaja: WILEY
Julkaisuvuosi: 2021
Journal: Molecular Nutrition and Food Research
Tietokannassa oleva lehden nimi: MOLECULAR NUTRITION & FOOD RESEARCH
Lehden akronyymi: MOL NUTR FOOD RES
Artikkelin numero: ARTN 2000898
Sivujen määrä: 11
ISSN: 1613-4125
eISSN: 1613-4133
DOI: https://doi.org/10.1002/mnfr.202000898
Verkko-osoite: https://onlinelibrary.wiley.com/doi/epdf/10.1002/mnfr.202000898
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/53651006
Scope: Structurally stable acylated anthocyanins have potential in various food applications but the effects of acylation and methoxysubstitution on anthocyanin metabolism are poorly understood. This is the first study thoroughly investigating phenolic metabolites, their time-wise changes, and pharmacokinetics following an acute intake of methoxysubstituted monoacylated anthocyanins.Methods and Results: Healthy male volunteers (n = 17) consumed a yellow potato meal with and without purple potato extract rich in acylated anthocyanins (152 mg) and hydroxycinnamic acid conjugates (140 mg). Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) is used for identification and quantification of metabolites from serially collected urine and plasma. While the parent anthocyanins are not detected, 28 phenolic metabolites from urine and 14 from plasma are quantified, including hydroxybenzoic and hydroxycinnamic acids and protocatechuic acid sulfates and glucuronides; three (catechol, gallic acid-4-O-glucuronide, and 2-methoxybenzoic acid) are detected for the first time after anthocyanin-rich food. Urinary hippuric acid is the most abundant with an increase of 139 mu M mM(-1) creatinine after the treatment. A large additional set of tentatively identified phenolic metabolites are detected. Late urinary peak time values suggest colonic degradation.Conclusion: Acylated anthocyanins are more bioavailable than earlier reported after extensive degradation in human and/or colonial metabolism to phenolic metabolites, which may be further conjugated and demethylated.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
