A1 Refereed original research article in a scientific journal
Interaction of storage medium and silver diamine fluoride on demineralized dentin
Authors: Peng Jing-Yuan, Botelho Michael George, Matinlinna Jukka Pekka, Pan Hao-Bo, Kukk Edwin, Low Kam-Jung
Publisher: SAGE PUBLICATIONS LTD
Publication year: 2021
Journal: Journal of International Medical Research
Journal name in source: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
Journal acronym: J INT MED RES
Article number: ARTN 0300060520985336
Volume: 49
Issue: 2
Number of pages: 12
ISSN: 0300-0605
eISSN: 1473-2300
DOI: https://doi.org/10.1177/0300060520985336
Web address : https://journals.sagepub.com/doi/full/10.1177/0300060520985336
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/53630798
Objective: The effects of saliva on demineralized dentin and silver diamine fluoride (SDF) were investigated in vitro.Methods: Dentin samples stored in deionized water (DIW), buffer solution (BS), basal medium mucin (BMM), and unstimulated whole saliva (UWS) were demineralized for 3 days and immersed in the same storage media. SDF as a 38 mass% solution was applied to the dentin samples for 3 minutes after they had been replaced in their respective medium. Surfaces were analyzed by scanning electron microscopy, energy-dispersive X-ray analysis (EDX), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD).Results: Scanning electron microscopy showed various surface deposits and coatings, including occlusion of dentinal tubules. DIW resulted in the thinnest coating, whereas BMM resulted in the thickest. EDX and XPS showed the formation of metallic silver and silver compounds in all four media, with the greatest formation in BS. XRD indicated that the main product was silver chloride except in DIW. Sulphur was found in BMM and UWS. EDX and XPS detected fluoride and XRD detected calcium fluoride and fluorohydroxyapatite in BS, BMM, and UWS.ConclusionThe interaction between SDF and demineralized dentin was dependent upon the storage medium. BMM provided an outcome most similar to human saliva.
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