The implementation of various neutrophil activities has been recently applied in toxicological assessments. The generation of a series of reactive oxygen species is a key event in the phagocytosis activity of neutrophils. These processes generate photon emission, chemiluminescence (CL). This native CL can be amplified with luminol. Zymosan, a cell wall preparate of a yeast Saccharomyces cerevisiae, opsonized in serum, provides a very strong activating effect on neutrophils and an intense luminol-amplified CL signal emerges. When, in addition to opsonized zymosan, toxic samples were added to the reaction mixture the CL signal was dose-dependently reduced. An EC50 value was determined from the dose curve where the CL signal was reduced 50 %. An indoor dust sample extracted with water from a moisture-damaged object had an EC50 value of 72 μg/ml in the neutrophil toxicity test. Testing in vivo indoor air toxicity on humans starts in spring 2016. Here were present toxicity experiments with test animals.