A1 Refereed original research article in a scientific journal
Cleavage of an RNA Model Compound by an Arylmercury Complex
Authors: Saleh Lange Yakubu, Ora Mikko, Lönnberg Tuomas
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2021
Journal: ChemBioChem
Journal name in source: CHEMBIOCHEM
Journal acronym: CHEMBIOCHEM
Number of pages: 5
ISSN: 1439-4227
eISSN: 1439-7633
DOI: https://doi.org/10.1002/cbic.202000799
Web address : https://doi.org/10.1002/cbic.202000799
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/53396727
Abstract
A water-soluble arylmercury complex has been synthesized, and its ability to catalyze the cleavage of the phosphodiester linkage of the RNA model compound adenylyl-3 ',5 '-(2 ',3 '-O-methyleneadenosine) has been assessed over a pH range of 3-8.5 and a catalyst concentration range of 0-7 mM. In the presence of 1 mM catalyst, the observed pH-rate profile featured a new pH-independent region between pH 6 and 7, the catalyzed reaction being as much as eight times faster than the background reaction. At pH 7, the acceleration increased linearly from three- to 17-fold upon increasing the catalyst concentration from 1 to 7 mM. The linear dependence indicates a relatively low affinity of the catalyst for the substrate and, hence, the potential for considerable improvement on tethering to an appropriate targeting group, such as an oligonucleotide.
A water-soluble arylmercury complex has been synthesized, and its ability to catalyze the cleavage of the phosphodiester linkage of the RNA model compound adenylyl-3 ',5 '-(2 ',3 '-O-methyleneadenosine) has been assessed over a pH range of 3-8.5 and a catalyst concentration range of 0-7 mM. In the presence of 1 mM catalyst, the observed pH-rate profile featured a new pH-independent region between pH 6 and 7, the catalyzed reaction being as much as eight times faster than the background reaction. At pH 7, the acceleration increased linearly from three- to 17-fold upon increasing the catalyst concentration from 1 to 7 mM. The linear dependence indicates a relatively low affinity of the catalyst for the substrate and, hence, the potential for considerable improvement on tethering to an appropriate targeting group, such as an oligonucleotide.
Downloadable publication This is an electronic reprint of the original article. |  |
UTU Research Portal