Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes



: Prabhakar Neeraj, Belevich Ilya, Peurla Markus, Heiligenstein Xavier, Chang Huan-Cheng, Sahlgren Cecilia, Jokitalo Eija, Rosenholm Jessica M

Publisher: MDPI

: 2021

: Nanomaterials

: NANOMATERIALS

: NANOMATERIALS-BASEL

: ARTN 14

: 11

: 1

: 10

: 2079-4991

DOI: https://doi.org/10.3390/nano11010014

: https://www.mdpi.com/2079-4991/11/1/14

: https://research.utu.fi/converis/portal/Publication/53003270


Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.

Last updated on 2024-26-11 at 12:50