A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist
Tekijät: Zhang Lijian, Zhuang Xiaoqing, Kotitalo Päivi, Keller Thomas, Krzyczmonik Anna, Haaparanta-Solin Merja, Solin Olof, Forsback Sarita, Grönroos Tove J, Han Chunlei, López-Picón Francisco R, Xia Hechun
Kustantaja: IVYSPRING INT PUBL
Julkaisuvuosi: 2021
Journal: Theranostics
Tietokannassa oleva lehden nimi: THERANOSTICS
Lehden akronyymi: THERANOSTICS
Vuosikerta: 11
Numero: 3
Aloitussivu: 1147
Lopetussivu: 1161
Sivujen määrä: 15
ISSN: 1838-7640
eISSN: 1838-7640
DOI: https://doi.org/10.7150/thno.52197
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/52789673
Rationale: Olfactory ensheathing cell (OEC) transplantation has emerged as a promising therapy for spinal cord injury (SCI) repair. In the present study, we explored the possible mechanisms of OECs transplantation underlying neuroinflammation modulation.Methods: Spinal cord inflammation after intravenous OEC transplantation was detected in vivo and ex vivo by translocator protein PET tracer [F-18]F-DPA. To track transplanted cells, OECs were transduced with enhanced green fluorescent protein (eGFP) and HSV1-39tk using lentiviral vector and were monitored by fluorescence imaging and [F-18]FHBG study. Protein microarray analysis and ELISA studies were employed to analyze differential proteins in the injured spinal cord after OEC transplantation. The anti-inflammation function of the upregulated protein was also proved by in vitro gene knocking down experiments and OECs/microglia co-culture experiment.Results: The inflammation in the spinal cord was decreased after OEC intravenous transplantation. The HSV1-39tk-eGFP-transduced OECs showed no accumulation in major organs and were found at the injury site. After OEC transplantation, in the spinal cord tissues, the interleukin-1 receptor antagonist (IL-1Ra) was highly upregulated while many chemokines, including pro-inflammatory chemokines IL-1 alpha, IL-1 beta were downregulated. In vitro studies confirmed that lipopolysaccharide (LPS) stimulus triggered OECs to secrete IL-1Ra. OECs significantly suppressed LPS-stimulated microglial activity, whereas IL-1Ra gene knockdown significantly reduced their ability to modulate microglial activity.Conclusion: The OECs that reached the lesion site were activated by the release of pro-inflammatory cytokines from activated microglia in the lesion site and secreted IL-1Ra to reduce neuroinflammation. Intravenous transplantation of OECs has high therapeutic effectiveness for the treatment of SCI via the secretion of IL-1Ra to reduce neuroinflammation.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
