A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants
Tekijät: Lin GL, Golubchik T, Drysdale S, O'Connor D, Jefferies K, Brown A, de Cesare M, Bonsall D, Ansari MA, Aerssens J, Bont L, Openshaw P, Martinon-Torres F, Bowden R, Pollard AJ; RESCEU Investigators
Kustantaja: OXFORD UNIV PRESS INC
Julkaisuvuosi: 2020
Journal: Journal of Infectious Diseases
Tietokannassa oleva lehden nimi: JOURNAL OF INFECTIOUS DISEASES
Lehden akronyymi: J INFECT DIS
Vuosikerta: 222
Numero: supplement 7
Aloitussivu: S666
Lopetussivu: S671
Sivujen määrä: 6
ISSN: 0022-1899
eISSN: 1537-6613
DOI: https://doi.org/10.1093/infdis/jiaa448
Verkko-osoite: https://doi.org/10.1093/infdis/jiaa448
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development.
