A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Photosynthetic engineered living materials incorporating recombinant Chlamydomonas reinhardtii enable long-term semi-continuous photobiotransformation
Tekijät: Siitonen, Vilja; Siivola, Tiia; Napaumpaiporn, Pornpan; Tammelin, Tekla; Allahverdiyeva, Yagut
Kustantaja: Elsevier BV
Julkaisuvuosi: 2026
Lehti: New Biotechnology
Vuosikerta: 93
Aloitussivu: 316
Lopetussivu: 324
ISSN: 1871-6784
eISSN: 1876-4347
DOI: https://doi.org/10.1016/j.nbt.2026.04.004
Julkaisun avoimuus kirjaamishetkellä: Avoimesti saatavilla
Julkaisukanavan avoimuus : Kokonaan avoin julkaisukanava
Verkko-osoite: https://doi.org/10.1016/j.nbt.2026.04.004
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/523158704
Rinnakkaistallenteen lisenssi: CC BY
Rinnakkaistallennetun julkaisun versio: Kustantajan versio
Photosynthetic engineered living materials (ELMs) present an attractive platform for producing valuable compounds and fuels using solar energy. Compared to traditional suspension cultures, ELMs enable long-term biocatalytic activity, more efficient light utilization, and facilitate downstream processing. In this study, we developed photosynthetic ELMs by entrapping Chlamydomonas reinhardtii in either alginate or TEMPO-oxidized cellulose nanofibers (TCNF). The strain utilized was converting cyclohexanone to ε-caprolactone via photobiotransformation. The cell loading and matrix material were evaluated in short-term reactions and during a semi-continuous bioproduction in vials. We showed that the entrapped cells remain photosynthetically active and catalytically competent over extended periods. By replenishing the substrate and collecting the product every 24 h we achieved semi-continuous photobiotransformation for over two weeks, reaching an average productivity of 2.31 ± 0.26 g m−2 d−1 and accumulating 0.31 ± 0.03 mol m−2 corresponding to 3.49 ± 0.31 g L−1 of ε-caprolactone. These findings establish photosynthetic ELMs as a viable approach for long-term whole-cell photobiotransformation.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
This work was supported by the EU FET Open project “FuturoLEAF” (grant agreement no. 899576), the Research Council of Finland “AlgaLEAF” (project no. 322752 and 322754), the Novo Nordisk Foundation project “PhotoCat” (project no. NNF20OC0064371 to YA) and Jane and Aatos Erkko Foundation project “PhotoFactory” (project no. 230042 to YA); and the Research Council of Finland’s Flagship Programme (Competence Centre for Materials Bioeconomy, FinnCERES). The work was carried out using the PHOTOSYN infrastructure at the University of Turku.
