B1 Non-refereed article in a scientific journal
Single-Cell Transcriptomics of Human Lymph Node Stroma
Authors: Takeda Akira, Jalkanen Sirpa
Publication year: 2020
Journal: STAR protocols
Journal name in source: STAR protocols
Journal acronym: STAR Protoc
Article number: 100021
Volume: 1
Issue: 1
ISSN: 2666-1667
eISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2020.100021(external)
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/52191243(external)
Abstract
Accumulating evidence indicates that the immune system is regulated not only by immune cells but also by stromal cells in the tissue microenvironment. Characterization of non-hematopoietic cells has not been performed in depth, since markers of the subsets are limited. Recent advances of single-cell technology allow researchers to characterize comprehensively the heterogeneity of stromal cells in an unbiased manner. In this article, we provide step-by-step protocols for cell preparation for single-cell RNA sequencing to characterize the heterogeneity of stroma in human lymph nodes. For complete details on the use and execution of this protocol, please refer to Takeda et al. (2019).
Accumulating evidence indicates that the immune system is regulated not only by immune cells but also by stromal cells in the tissue microenvironment. Characterization of non-hematopoietic cells has not been performed in depth, since markers of the subsets are limited. Recent advances of single-cell technology allow researchers to characterize comprehensively the heterogeneity of stromal cells in an unbiased manner. In this article, we provide step-by-step protocols for cell preparation for single-cell RNA sequencing to characterize the heterogeneity of stroma in human lymph nodes. For complete details on the use and execution of this protocol, please refer to Takeda et al. (2019).
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