The blue gum chalcid (Leptocybe invasa) is a serious invasive, galling insect pest of eucalypts grown outside Australia. Variability in resistance of species and genotypes of Eucalyptus to the pest is widely reported but without consideration of the influence of silviculture on the severity of galling. We assessed the variability of gall expression by 29 genotypes of E. camaldulensis by L. invasa in common nursery experiments and in 5 common garden arboreta planted in diverse climatic zones and soil types around Kenya. We quantified variation in growth and the concentrations of defensive chemical compounds (namely polyphenolic compounds) to assess possible genotype × environment interactions which we also relate to the climate of the parent seed trees in Australia. Generally, genotypes endemic to low latitude regions of Australia were more resistant to the pest while the concentration of quinic acid derivatives (QUIN) exhibited an interaction with arboretum location in Kenya. The concentration of QUIN in potted plants did not vary significantly with nitrogen supplementation. However, growth rates and total polyphenolic concentrations varied with arboretum location. Since QUIN, which have been previously shown to confer resistance against L. invasa, did not vary in different arboreta, resistant subspecies and genotypes of E. camaldulensis can be deployed in novel habitats and will not be galled. Our findings support the critical need to plant stock of known genotype(s) rather than planting stock grown from locally collected seed. This will require the establishment of eucalypt seed orchards if clonal production of planting stock is not possible.