A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Covalently Mercurated Molecular Beacon for Discriminating the Canonical Nucleobases
Tekijät: Aro-Heinilä Asmo, Lönnberg Tuomas, Virta Pasi
Kustantaja: WILEY-V C H VERLAG GMBH
Julkaisuvuosi: 2021
Journal: ChemBioChem
Tietokannassa oleva lehden nimi: CHEMBIOCHEM
Lehden akronyymi: CHEMBIOCHEM
Vuosikerta: 22
Numero: 2
Aloitussivu: 354
Lopetussivu: 358
Sivujen määrä: 6
ISSN: 1439-4227
eISSN: 1439-7633
DOI: https://doi.org/10.1002/cbic.202000575
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/50712742
A highly nucleobase-discriminating metalated nucleoside analogue, 3-fluoro-2-mercuri-6-methylaniline, was incorporated into an oligonucleotide molecular beacon. Fluorescence emission spectra were measured after the addition of four different complementary strands, in which the nucleobase opposite the metalated analogue varies. The fluorescence results showed a clear binding selectivity at room temperature, in the order G>T>C>A. The selectivity is based on the different affinities between the metalated nucleoside analogue and the canonical nucleobases. The synthesized probe is capable of robust discrimination between the two purine as well as the two pyrimidine bases by fluorescence at room temperature, and more sophisticated temperature analysis allows clear separation of every canonical nucleobase. The probe would, hence, be a suitable method for the detection of single nucleotide polymorphisms.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
