A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Nanotopography-driven changes in focal adhesion morphology of human dermal fibroblast cells on inkjet-printed hierarchically structured hemitoroids
Tekijät: Rosqvist, Emil; Fogde, Anna; Fazeli, Elnaz; Belaya, Irina; Niemelä, Erik; Määttänen, Anni; Venu, Arun P.; Kankaanpää, Pasi; Eriksson, John E.; Peltonen, Jouko
Kustantaja: Elsevier BV
Julkaisuvuosi: 2025
Lehti: Materials and Design
Artikkelin numero: 114920
Vuosikerta: 259
ISSN: 0264-1275
eISSN: 1873-4197
DOI: https://doi.org/10.1016/j.matdes.2025.114920
Julkaisun avoimuus kirjaamishetkellä: Avoimesti saatavilla
Julkaisukanavan avoimuus : Kokonaan avoin julkaisukanava
Verkko-osoite: https://www.sciencedirect.com/science/article/pii/S0264127525013401?via%3Dihub
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/505767299
Two-component polymer particle dispersions can be used to make nanostructured coatings with diverse applications. In this study, blends of polystyrene and acrylonitrile butadiene styrene latex dispersions were formulated for inkjet printing. When printed onto glass coverslips with a 50 µm drop spacing (DS) the result was ultrathin, nanostructured coatings. Increasing the DS to 100 µm produced arrays of nanostructured hemitoroids approximately 30–60 µm in diameter. By adjusting the blend ratio, the nanotopography of the hemitoroids could be tuned (e.g. Sq 6–32 nm).
These hemitoroid arrays, which exhibited different nanotopographies, were used for studying the influence of nanotopography on the morphology of focal adhesions (FAs) of human dermal fibroblast cells. Statistically significant differences in FA shape (e.g. roundness and aspect ratio) and size (area and perimeter length) were observed for different latex blends — even when the difference in RMS roughness (Sq) was only 3.9 nm. Strong correlations were observed between FA morphology and surface roughness in terms of Sa, Sq, Sdr, Spk, and Sk (R2 ≥ 0.9 and Pearson’s r ≥ |0.95|), indicating that the response in FA morphology was mainly driven by nanoscale height variations and fine texture of these surfaces. These findings hence highlight the role of nanotopography in modulating substrate-cell signal transduction.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
Rosqvist acknowledges funding from Jane and Aatos Erkko foundation (project: Anti-Bacterial Channelling from Waste to Human Health), Tor, Joe och Pentti Borgs Minnesfond, and Waldemar von Frenckells stiftelse. This research was commenced as part of the Functional Materials at Biological Interfaces Centre of Excellence at Åbo Akademi University.
For service, instrumentation, and expertise we would like to thank the Cell Imaging and Cytometry Core (Turku Bioscience Centre, Turku, Finland) and Turku BioImaging Image Data Team (Turku, Finland), which are part of the Finnish Advanced Microscopy Node of EuroBioImaging Finland funded by the Research Council of Finland, FIRI grant numbers 328040, 328044.
Parts of the research carried out in this work used Research Council of Finland Research Infrastructure “Printed Intelligence Infrastructure” (PII-FIRI).
