It is essential to exploit the eggshell membrane (EM) and eggshell (ES) for biomaterial science as a bioresource technology albeit that they are generally considered as waste products of the egg industry. The EM with Janus structural and compositional properties can be prepared into tissue-engineered constructs. The ES was prepared into the inorganic nanoparticles (NPs) namely PCa with an average diameter of 0.67 μm, which was comprised of various inorganic oxides, such as calcium oxide (CaO), and zinc oxide (ZnO). By harnessing the individual advantages of the EM and PCa, they were fabricated into composite scaffolds by a negative pressure inlay method. The composite scaffolds manifested a fibrous network-like structure manifesting large surface area, good mechanical strength (failure force of EM, ca. 1.68 N), biocompatibility and biodegradability in vitro and in vivo. The EM@Ca3 group enabled efficient hemostasis in the liver trauma injury model (hemostasis time, <32 s), rapid wound healing (96 % at day 14), and bone density similar to the normal bone at week 6 post-implantation. Taken together, our approach of leveraging egg-derived bioresource may be worthy for the future investigations of tissue-engineered constructs and potentially other bio-related disciplines.