A1 Refereed original research article in a scientific journal
Characterization of Highly Mucus-Adherent Non-GMO Derivatives of Lacticaseibacillus rhamnosus GG
Authors: Pia Rasinkangas, Hanne L.P. Tytgat, Jarmo Ritari, Justus Reunanen, Seppo Salminen, Aira Palva, Francois P. Douillard, Willem M. de Vos
Publisher: FRONTIERS MEDIA SA
Publication year: 2020
Journal: Frontiers in Bioengineering and Biotechnology
Journal name in source: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Journal acronym: FRONT BIOENG BIOTECH
Article number: ARTN 1024
Volume: 8
Number of pages: 11
ISSN: 2296-4185
eISSN: 2296-4185
DOI: https://doi.org/10.3389/fbioe.2020.01024
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/50371217
Lacticaseibacillus rhamnosusGG is one of the best studied lactic acid bacteria in the context of probiotic effects.L. rhamnosusGG has been shown to prevent diarrhea in children and adults and has been implicated to have mitigating or preventive effects in several disorders connected to microbiota dysbiosis. The probiotic effects are largely attributed to its adhesive heterotrimeric sortase-dependent pili, encoded by thespaCBA-srtC1gene cluster. Indeed, the strain-specific SpaCBA pili have been shown to contribute to adherence, biofilm formation and host signaling. In this work we set out to generate non-GMO derivatives ofL. rhamnosusGG that adhere stronger to mucus compared to the wild-type strain using chemical mutagenesis. We selected 13 derivatives that showed an increased mucus-adherent phenotype. Deep shotgun resequencing of the strains enabled division of the strains into three classes, two of which revealed SNPs (single nucleotide polymorphisms) in thespaAandspaCgenes encoding the shaft and tip adhesive pilins, respectively. Strikingly, the other class derivatives demonstrated less clear genotype - phenotype relationships, illustrating that pili biogenesis and structure is also affected by other processes. Further characterization of the different classes of derivatives was performed by PacBio SMRT sequencing and RNAseq analysis, which resulted in the identification of molecular candidates driving pilin biosynthesis and functionality. In conclusion, we report on the generation and characterization of three classes of strongly adherentL. rhamnosusGG derivatives that show an increase in adhesion to mucus. These are of special interest as they provide a window on processes and genes driving piliation and its control inL. rhamnosusGG and offer a variety of non-GMO derivatives of this key probiotic strain that are applicable in food products.
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