A1 Refereed original research article in a scientific journal
Cultivation and Genomics Prove Long-Term Colonization of Donor's Bifidobacteria in Recurrent Clostridioides difficile Patients Treated With Fecal Microbiota Transplantation
Authors: Hanne Jouhten, Aki Ronkainen, Juhani Aakko, Seppo Salminen, Eero Mattila, Perttu Arkkila, Reetta Satokari
Publisher: FRONTIERS MEDIA SA
Publication year: 2020
Journal: Frontiers in Microbiology
Journal name in source: FRONTIERS IN MICROBIOLOGY
Journal acronym: FRONT MICROBIOL
Article number: ARTN 1663
Volume: 11
Number of pages: 13
ISSN: 1664-302X
eISSN: 1664-302X
DOI: https://doi.org/10.3389/fmicb.2020.01663
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/49897275
Fecal microbiota transplantation (FMT) is an effective treatment for recurrentClostridioides difficileinfection (rCDI) and it's also considered for treating other indications. Metagenomic studies have indicated that commensal donor bacteria may colonize FMT recipients, but cultivation has not been employed to verify strain-level colonization. We combined molecular profiling ofBifidobacteriumpopulations with cultivation, molecular typing, and whole genome sequencing (WGS) to isolate and identify strains that were transferred from donors to recipients. SeveralBifidobacteriumstrains from two donors were recovered from 13 recipients during the 1-year follow-up period after FMT. The strain identities were confirmed by WGS and comparative genomics. Our results show that specific donor-derived bifidobacteria can colonize rCDI patients for at least 1 year, and thus FMT may have long-term consequences for the recipient's microbiota and health. Conceptually, we demonstrate that FMT trials combined with microbial profiling can be used as a platform for discovering and isolating commensal strains with proven colonization capacity for potential therapeutic use.
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