New taxonomic and faunistic data on the dysderid spiders of Kosovo are presented, along with a survey of previous records. Harpactea dardanica Geci & Zamani sp. nov., belonging to the lepida species-group, is described as new to science based on male specimens collected in the Bjeshkët-e-Nemuna National Park, western Kosovo. Dysdera adriatica Kulczyński, 1897, Dy. lata Reuss, 1834, Dasumia kusceri (Kratochvíl, 1935), H. rubicunda (C.L. Koch, 1838), H. srednagora Dimitrov & Lazarov, 1999, and H. tenuiemboli Deltshev, 2011, as well as the genus Dasumia Thorell, 1875, are reported from Kosovo for the first time. Additional records are provided for Dy. longirostris Doblika, 1853 and H. lepida (C.L. Koch, 1838). DNA barcodes (partial sequences of the mitochondrial cytochrome c oxidase subunit 1 gene) were generated for all species. Photographic illustrations are provided for the newly described species, as well as for H. srednagora and H. tenuiemboli.