Protein Structure Analysis and Validation with X-Ray Crystallography

: Papageorgiou Anastasios C, Poudel Nirmal, Mattsson Jesse

: Labrou Nikolaos E.

: 2nd

: 2021

: Methods in Molecular Biology

: Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods

: Methods in molecular biology (Clifton, N.J.)

: Methods Mol Biol

: Methods in Molecular Biology

: 2178

: 377

: 404

: 978-1-0716-0774-9

: 978-1-0716-0775-6

: 1064-3745

: 1940-6029

DOI: https://doi.org/10.1007/978-1-0716-0775-6_25

X-ray crystallography is the main technique for the determination of protein structures. About 85% of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design , and basic research and as a validation tool for protein modifications and ligand binding. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.