A3 Refereed book chapter or chapter in a compilation book
Protein Structure Analysis and Validation with X-Ray Crystallography
Authors: Papageorgiou Anastasios C, Poudel Nirmal, Mattsson Jesse
Editors: Labrou Nikolaos E.
Edition: 2nd
Publication year: 2021
Journal: Methods in Molecular Biology
Book title : Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods
Journal name in source: Methods in molecular biology (Clifton, N.J.)
Journal acronym: Methods Mol Biol
Series title: Methods in Molecular Biology
Volume: 2178
First page : 377
Last page: 404
ISBN: 978-1-0716-0774-9
eISBN: 978-1-0716-0775-6
ISSN: 1064-3745
eISSN: 1940-6029
DOI: https://doi.org/10.1007/978-1-0716-0775-6_25
Abstract
X-ray crystallography is the main technique for the determination of protein structures. About 85% of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design , and basic research and as a validation tool for protein modifications and ligand binding. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.
X-ray crystallography is the main technique for the determination of protein structures. About 85% of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design , and basic research and as a validation tool for protein modifications and ligand binding. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.
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