A1 Refereed original research article in a scientific journal
Development of a bioluminescent cyanobacterial reporter strain for detection of arsenite, arsenate and antimonite
Authors: Peca L, Nagy CI, Ordog A, Vass I, Kos PB
Publisher: GH ASACHI TECHNICAL UNIV IASI
Publication year: 2017
Journal: Environmental Engineering and Management Journal
Journal name in source: ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL
Journal acronym: ENVIRON ENG MANAG J
Volume: 16
Issue: 11
First page : 2443
Last page: 2450
Number of pages: 8
ISSN: 1582-9596
eISSN: 1843-3707
Web address : http://eemj.eu/index.php/EEMJ/article/view/3408
Abstract
In the present study the potential of a gene fusion between the arsB promoter from Synechocystis 6803 and the bacterial luxAB genes was evaluated to be used as a cyanobacterial bioreporter for monitoring the bioavailability of inorganic arsenic species. A whole-cell bioreporter strain, designated arsLux, was constructed based on this fusion. In concert with the specificity of the promoter presented earlier, luminescent signal could be detected upon exposure to arsenite, arsenate and antimonite, in a concentration-dependent manner, following an incubation period of 14 hours. The detection range of arsLux was 4 mu M to 1 mM for As(III) and Sb(III), and 150 mu M to 150 mM for As(V). However, arsLux activity was inhibited by Cu2+ and Zn2+ with a half maximal inhibitory concentration (IC50) of about 8 mu M and 16 mu M, respectively. The bioreporter performance was tested using water samples from a thermal spring and from the River Tisza, both of them supplemented with arsenite. In the first case the bioluminescent signal was comparable with the signal of the standard solution, whereas in the second case the signal was much lower, presumably due to inhibitors present in the river water. Our data show the arsB promoter has the potential for whole cell bioreporter applications with some further improvements that are also discussed.
In the present study the potential of a gene fusion between the arsB promoter from Synechocystis 6803 and the bacterial luxAB genes was evaluated to be used as a cyanobacterial bioreporter for monitoring the bioavailability of inorganic arsenic species. A whole-cell bioreporter strain, designated arsLux, was constructed based on this fusion. In concert with the specificity of the promoter presented earlier, luminescent signal could be detected upon exposure to arsenite, arsenate and antimonite, in a concentration-dependent manner, following an incubation period of 14 hours. The detection range of arsLux was 4 mu M to 1 mM for As(III) and Sb(III), and 150 mu M to 150 mM for As(V). However, arsLux activity was inhibited by Cu2+ and Zn2+ with a half maximal inhibitory concentration (IC50) of about 8 mu M and 16 mu M, respectively. The bioreporter performance was tested using water samples from a thermal spring and from the River Tisza, both of them supplemented with arsenite. In the first case the bioluminescent signal was comparable with the signal of the standard solution, whereas in the second case the signal was much lower, presumably due to inhibitors present in the river water. Our data show the arsB promoter has the potential for whole cell bioreporter applications with some further improvements that are also discussed.
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