A1 Refereed original research article in a scientific journal
Multiplexed High-Throughput Serological Assay for Human Enteroviruses
Authors: Saarinen NVV, Lehtonen J, Veijola R, Lempainen J, Knip M, Hyöty H, Laitinen OH, Hytönen VP
Publisher: MDPI
Publication year: 2020
Journal: Microorganisms
Journal name in source: MICROORGANISMS
Journal acronym: MICROORGANISMS
Article number: ARTN 963
Volume: 8
Issue: 6
Number of pages: 16
eISSN: 2076-2607
DOI: https://doi.org/10.3390/microorganisms8060963
Web address : https://doi.org/10.3390/microorganisms8060963
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/49435006
Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Seven recombinant VP1 proteins from enteroviruses EV-A to EV-D and rhinoviruses RV-A to RV-C species were produced. Using Meso Scale Diagnostics U-PLEX platform we were able to study antibody reactions against these proteins as well as non-structural enterovirus proteins in a single well with 140 human serum samples. Adults had on average 33-fold stronger antibody responses to these antigens (p< 10(-11)) compared to children, but children had less cross-reactivity between different enterovirus types. The results suggest that this new high-throughput assay offers clear benefits in the evaluation of humoral enterovirus immunity in children, giving more exact information than assays that are based on a single enterovirus type as antigen.
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