An autoradiographic study of [F-18]FDG uptake to islets of Langerhans in NOD mouse

: Kalliokoski T, Simell O, Haaparanta M, Vijanen T, Solin O, Knuuti J, Nuutila P

Publisher: ELSEVIER IRELAND LTD

: 2005

: Diabetes Research and Clinical Practice

: DIABETES RESEARCH AND CLINICAL PRACTICE

: DIABETES RES CLIN PR

: 70

: 3

: 217

: 224

: 8

: 0168-8227

DOI: https://doi.org/10.1016/j.diabres.2005.04.008

To evaluate the potential of in vivo imaging of accumulation of lymphocytes to islets of Langerhans (insulitis), we compared 2-[F-18]fluoro-2-deoxy-D-glucose ([F-18]FDG) uptake in the pancreas and pancreatic islets of healthy BALB/c mice, phenotypically healthy NOD mice with insulitis and diabetic NOD mice. [F-18]FDG was injected i.v. to 14 female BALB/c mice (age 13 3 weeks, plasma glucose 8 +/- 2mmol/1) and 21 age-matched female NOD mice (plasma glucose 8 +/- 4 mmol/l, p = 0.06). The mice were killed 90-min post injection and distribution of radioactivity was analysed using digital autoradiography. There was no correlation of plasma glucose concentration with the [(18) F]FDG uptake values. Uptake of radioactivity in NOD mice to the islets affected by insulitis was up to 2.3 times higher (p = 0.001) than that to unaffected islets in the same pancreas. Uptake to NOD islets with insulitis was also clearly enhanced (1.0-2.3 times higher) compared to the islets in the BALB/c mice. In conclusion, NOD mouse islets with insulitis accumulate [F-18]FDG markedly more than islets without insulitis or BALB/c islets. However, the relatively small difference in the [F-18]FDG intensity between healthy and diseased islets, combined with the limited resolution ability of the positron emission tomography (PET), probably prevent the use of [F-18]FDG in PET studies aiming at in vivo documentation of onset and progression of insulitis and prediabetes in mouse and man. (c) 2005 Elsevier Ireland Ltd. All rights reserved.