A1 Refereed original research article in a scientific journal
An autoradiographic study of [F-18]FDG uptake to islets of Langerhans in NOD mouse
Authors: Kalliokoski T, Simell O, Haaparanta M, Vijanen T, Solin O, Knuuti J, Nuutila P
Publisher: ELSEVIER IRELAND LTD
Publication year: 2005
Journal: Diabetes Research and Clinical Practice
Journal name in source: DIABETES RESEARCH AND CLINICAL PRACTICE
Journal acronym: DIABETES RES CLIN PR
Volume: 70
Issue: 3
First page : 217
Last page: 224
Number of pages: 8
ISSN: 0168-8227
DOI: https://doi.org/10.1016/j.diabres.2005.04.008
Abstract
To evaluate the potential of in vivo imaging of accumulation of lymphocytes to islets of Langerhans (insulitis), we compared 2-[F-18]fluoro-2-deoxy-D-glucose ([F-18]FDG) uptake in the pancreas and pancreatic islets of healthy BALB/c mice, phenotypically healthy NOD mice with insulitis and diabetic NOD mice. [F-18]FDG was injected i.v. to 14 female BALB/c mice (age 13 3 weeks, plasma glucose 8 +/- 2mmol/1) and 21 age-matched female NOD mice (plasma glucose 8 +/- 4 mmol/l, p = 0.06). The mice were killed 90-min post injection and distribution of radioactivity was analysed using digital autoradiography. There was no correlation of plasma glucose concentration with the [(18) F]FDG uptake values. Uptake of radioactivity in NOD mice to the islets affected by insulitis was up to 2.3 times higher (p = 0.001) than that to unaffected islets in the same pancreas. Uptake to NOD islets with insulitis was also clearly enhanced (1.0-2.3 times higher) compared to the islets in the BALB/c mice. In conclusion, NOD mouse islets with insulitis accumulate [F-18]FDG markedly more than islets without insulitis or BALB/c islets. However, the relatively small difference in the [F-18]FDG intensity between healthy and diseased islets, combined with the limited resolution ability of the positron emission tomography (PET), probably prevent the use of [F-18]FDG in PET studies aiming at in vivo documentation of onset and progression of insulitis and prediabetes in mouse and man. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
To evaluate the potential of in vivo imaging of accumulation of lymphocytes to islets of Langerhans (insulitis), we compared 2-[F-18]fluoro-2-deoxy-D-glucose ([F-18]FDG) uptake in the pancreas and pancreatic islets of healthy BALB/c mice, phenotypically healthy NOD mice with insulitis and diabetic NOD mice. [F-18]FDG was injected i.v. to 14 female BALB/c mice (age 13 3 weeks, plasma glucose 8 +/- 2mmol/1) and 21 age-matched female NOD mice (plasma glucose 8 +/- 4 mmol/l, p = 0.06). The mice were killed 90-min post injection and distribution of radioactivity was analysed using digital autoradiography. There was no correlation of plasma glucose concentration with the [(18) F]FDG uptake values. Uptake of radioactivity in NOD mice to the islets affected by insulitis was up to 2.3 times higher (p = 0.001) than that to unaffected islets in the same pancreas. Uptake to NOD islets with insulitis was also clearly enhanced (1.0-2.3 times higher) compared to the islets in the BALB/c mice. In conclusion, NOD mouse islets with insulitis accumulate [F-18]FDG markedly more than islets without insulitis or BALB/c islets. However, the relatively small difference in the [F-18]FDG intensity between healthy and diseased islets, combined with the limited resolution ability of the positron emission tomography (PET), probably prevent the use of [F-18]FDG in PET studies aiming at in vivo documentation of onset and progression of insulitis and prediabetes in mouse and man. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
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