A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
The forgotten element: Why do we ignore calcium in otolith studies?
Tekijät: Limburg, Karin E.; Heimbrand, Yvette; Hussy, Karin; Blass, Martina; Thomas, Jay B.; Mäkinen, Katja; Naeraa, Tomas
Kustantaja: Elsevier BV
Kustannuspaikka: AMSTERDAM
Julkaisuvuosi: 2025
Journal: Fisheries Research
Tietokannassa oleva lehden nimi: Fisheries Research
Lehden akronyymi: FISH RES
Artikkelin numero: 107297
Vuosikerta: 283
Sivujen määrä: 7
ISSN: 0165-7836
eISSN: 1872-6763
DOI: https://doi.org/10.1016/j.fishres.2025.107297
Verkko-osoite: https://doi.org/10.1016/j.fishres.2025.107297
Typical analyses of otolith microchemistry use calcium, a major constituent, as an internal standard, setting its value as a constant and ignoring any potential variations. In fact, patterns do occur in otolith Ca deposition, as can be observed either by repeating the analysis, by creating two-dimensional maps of Ca, or both. Here we present evidence of Ca variations in fish otoliths from analyses using synchrotron-based scanning X-ray fluorescence microscopy, electron microprobe analysis, and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). 2-D maps of otoliths created with LA-ICP-MS indicate that Ca is elevated where especially Zn and P are low, and vice versa, suggesting that spatial variations in protein deposition may affect concentrations of Ca. We encourage others to examine Ca concentrations in their biomineralized samples to check for variations, using LA-ICP-MS and other methods.
Julkaisussa olevat rahoitustiedot:
This work is supported in part by a grant from the U.S. National Science Foundation (award OCE-1923965). The SXFM work took place at the F3 beamline at CHESS in 2017, supported by NSF award DMR-00936384. CHESS operations are currently supported by the National Science Foundation (BIO, ENG and MPS Directorates) under award DMR-1829070, by AFRL award FA8650-22-2-5200, by NIGMS award 1-P30-GM124166-01A1 and NYSTAR. National Science Foundation MRI grant EAR1625835 and support from Syracuse University facilitated measurements in the EPMA laboratory.
