A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation

Julkaisun tekijät: Willy V Bienvenut, Annika Brünje, Jean‐Baptiste Boyer, Jens S Mühlenbeck, Gautier Bernal, Ines Lassowskat, Cyril Dian, Eric Linster, Trinh V Dinh, Minna M Koskela, Vincent Jung, Julian Seidel, Laura K Schyrba, Aiste Ivanauskaite, Jürgen Eirich, Rüdiger Hell, Dirk Schwarzer, Paula Mulo, Markus Wirtz, Thierry Meinnel, Carmela Giglione, Iris Finkemeier

Kustantaja: WILEY

Julkaisuvuosi: 2020

Journal: Molecular Systems Biology

Tietokannassa oleva lehden nimi: MOLECULAR SYSTEMS BIOLOGY

Lehden akronyymi: MOL SYST BIOL

Volyymi: 16

Julkaisunumero: 7

Sivujen määrä: 23

ISSN: 1744-4292

eISSN: 1744-4292

DOI: http://dx.doi.org/10.15252/msb.20209464

Protein acetylation is a highly frequent protein modification. However, comparatively little is known about its enzymatic machinery. N-alpha-acetylation (NTA) and epsilon-lysine acetylation (KA) are known to be catalyzed by distinct families of enzymes (NATs andKATs, respectively), although the possibility that the sameGCN5-relatedN-acetyltransferase (GNAT) can perform both functions has been debated. Here, we discovered a new family of plastid-localizedGNATs, which possess a dual specificity. All characterizedGNATfamily members display a number of unique features. Quantitative mass spectrometry analyses revealed that these enzymes exhibit both distinctKAand relaxedNTAspecificities. Furthermore, inactivation ofGNAT2 leads to significantNTAorKAdecreases of several plastid proteins, while proteins of other compartments were unaffected. The data indicate that these enzymes have specific protein targets and likely display partly redundant selectivity, increasing the robustness of the acetylation processin vivo. In summary, this study revealed a new layer of complexity in the machinery controlling this prevalent modification and suggests that other eukaryoticGNATs may also possess these previously underappreciated broader enzymatic activities.

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Last updated on 2021-24-06 at 10:41