A1 Refereed original research article in a scientific journal
Influence of Cell Membrane Wrapping on the Cell-Porous Silicon Nanoparticle Interactions
Authors: Flavia Fontana, Hanna Lindstedt, Alexandra Correia, Jacopo Chiaro, Otto K. Kari, Joseph Ndika, Harri Alenius, Jonas Buck, Sandro Sieber, Ermei Mäkilä, Jarno Salonen, Arto Urtti, Vincenzo Cerullo, Jouni T. Hirvonen, Hélder A. Santos
Publisher: WILEY
Publication year: 2020
Journal: Advanced Healthcare Materials
Journal name in source: ADVANCED HEALTHCARE MATERIALS
Journal acronym: ADV HEALTHC MATER
Article number: ARTN 2000529
Volume: 9
Issue: 17
Number of pages: 9
ISSN: 2192-2640
DOI: https://doi.org/10.1002/adhm.202000529
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/48672980
Biohybrid nanosystems represent the cutting-edge research in biofunctionalization of micro- and nano-systems. Their physicochemical properties bring along advantages in the circulation time, camouflaging from the phagocytes, and novel antigens. This is partially a result of the qualitative differences in the protein corona, and the preferential targeting and uptake in homologous cells. However, the effect of the cell membrane on the cellular endocytosis mechanisms and time has not been fully evaluated yet. Here, the effect is assessed by quantitative flow cytometry analysis on the endocytosis of hydrophilic, negatively charged porous silicon nanoparticles and on their membrane-coated counterparts, in the presence of chemical inhibitors of different uptake pathways. Principal component analysis is used to analyze all the data and extrapolate patterns to highlight the cell-specific differences in the endocytosis mechanisms. Furthermore, the differences in the composition of static protein corona between naked and coated particles are investigated together with how these differences affect the interaction with human macrophages. Overall, the presence of the cell membrane only influences the speed and the entity of nanoparticles association with the cells, while there is no direct effect on the endocytosis pathways, composition of protein corona, or any reduction in macrophage-mediated uptake.
Downloadable publication This is an electronic reprint of the original article. |  |
