A2 Refereed review article in a scientific journal
Application of bacteriophages in sensor development
Authors: Peltomaa R, Lopez-Perolio I, Benito-Pena E, Barderas R, Moreno-Bondi MC
Publisher: SPRINGER HEIDELBERG
Publication year: 2016
Journal: Analytical and Bioanalytical Chemistry
Journal name in source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Journal acronym: ANAL BIOANAL CHEM
Volume: 408
Issue: 7
First page : 1805
Last page: 1828
Number of pages: 24
ISSN: 1618-2642
DOI: https://doi.org/10.1007/s00216-015-9087-2
Abstract
Bacteriophage-based bioassays are a promising alternative to traditional antibody-based immunoassays. Bacteriophages, shortened to phages, can be easily conjugated or genetically engineered. Phages are robust, ubiquitous in nature, and harmless to humans. Notably, phages do not usually require inoculation and killing of animals; and thus, the production of phages is simple and economical. In recent years, phage-based biosensors have been developed featuring excellent robustness, sensitivity, and selectivity in combination with the ease of integration into transduction devices. This review provides a critical overview of phage-based bioassays and biosensors developed in the last few years using different interrogation methods such as colorimetric, enzymatic, fluorescence, surface plasmon resonance, quartz crystal microbalance, magnetoelastic, Raman, or electrochemical techniques.
Bacteriophage-based bioassays are a promising alternative to traditional antibody-based immunoassays. Bacteriophages, shortened to phages, can be easily conjugated or genetically engineered. Phages are robust, ubiquitous in nature, and harmless to humans. Notably, phages do not usually require inoculation and killing of animals; and thus, the production of phages is simple and economical. In recent years, phage-based biosensors have been developed featuring excellent robustness, sensitivity, and selectivity in combination with the ease of integration into transduction devices. This review provides a critical overview of phage-based bioassays and biosensors developed in the last few years using different interrogation methods such as colorimetric, enzymatic, fluorescence, surface plasmon resonance, quartz crystal microbalance, magnetoelastic, Raman, or electrochemical techniques.
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