A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Nuclear talin-1 provides a bridge between cell adhesion and gene expression
Tekijät: Da Silva, Alejandro J.; Hästbacka, Hendrik S. E.; Puustinen, Mikael C.; Pessa, Jenny C.; Luoto, Jens C.; Sundström, Erika; Goult, Benjamin T.; Jacquemet, Guillaume; Henriksson, Eva; Sistonen, Lea
Kustantaja: Cell Press
Kustannuspaikka: CAMBRIDGE
Julkaisuvuosi: 2025
Journal: iScience
Tietokannassa oleva lehden nimi: iScience
Lehden akronyymi: ISCIENCE
Artikkelin numero: 111745
Vuosikerta: 28
Numero: 2
Aloitussivu: 111745
Sivujen määrä: 18
ISSN: 2589-0042
eISSN: 2589-0042
DOI: https://doi.org/10.1016/j.isci.2025.111745
Verkko-osoite: https://doi.org/10.1016/j.isci.2025.111745
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/485078218
Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. To determine the functional impact of nuclear TLN1, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our findings revealed that the accumulation of nuclear TLN1 alters the expression of a subset of genes and impairs the formation of cell-cell clusters. This study introduces an additional perspective on the canonical view of TLN1 subcellular localization and function.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
This work has been funded by the Research Council of Finland (338537 to G.J. and 355596 to L.S.), Sigrid Jusélius Foundation (G.J. and L.S.), Cancer Foundation Finland (G.J. and L.S.), Centre for International Mobility (A.J.D.S. and E.H.), Ella and Georg Ehrnrooth Foundation (A.J.D.S. and H.S.E.H.), Finnish Cultural Foundation (A.J.D.S.), K. Albin Johansson Foundation (A.J.D.S. and E.H.), InFLAMES Flagship Programme of the Research Council of Finland (decision numbers: 337530, 337531, 357910, and 357911 to G.J.), Magnus Ehrnrooth Foundation (A.J.D.S., J.C.L., and E.H.), Medicinska Understödsföreningen Liv och Hälsa r.f. (A.J.D.S., E.H., and L.S.), Otto A. Malm Foundation (A.J.D.S.), The Maud Kuistila Memorial Foundation (H.S.E.H.), Solutions for Health strategic funding to Åbo Akademi University (G.J.), and Åbo Akademi University (A.J.D.S., J.C.P., and L.S.).
