A1 Refereed original research article in a scientific journal
The interactome of the prostate-specific protein Anoctamin 7
Authors: Elina Kaikkonen, Aliisa Takala, Juha-Pekka Pursiheimo, Gudrun Wahlström, Johanna Schleutker
Publisher: IOS PRESS
Publication year: 2020
Journal: Cancer Biomarkers
Journal name in source: CANCER BIOMARKERS
Journal acronym: CANCER BIOMARK
Volume: 28
Issue: 1
First page : 91
Last page: 100
Number of pages: 10
ISSN: 1574-0153
eISSN: 1875-8592
DOI: https://doi.org/10.3233/CBM-190993
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/48474953
BACKGROUND: Elevated Anoctamin 7 (ANO7) expression is associated with poor survival in prostate cancer patients.OBJECTIVE: The aim was to discover proteins that interact with ANO7 to understand its functions and regulatory mechanisms.METHODS: The proximity-dependent biotin identification (BioID) method was utilized. ANO7 fused to biotin ligase was transiently transfected into LNCaP cells, and the biotinylated proteins were collected and analysed by mass spectrometry. Four identified proteins were stained with dual fluorescent immunostaining and visualized using Stimulated emission depletion microscopy (STED).RESULTS: After bioinformatic filtering steps, 64 potentially ANO7-interacting proteins were identified and analysed with the GO enrichment analysis tool. One of the most prominently enriched cellular components was cellular vesicle. Co-localization was showed for staphylococcal nuclease and tudor domain containing 1 (SND1), heat shock protein family A (Hsp70) member 1A (HSPA1A), adaptor related protein complex 2 subunit beta 1 (AP2B1) and coatomer protein complex subunit gamma 2 (COPG2).CONCLUSIONS: This is the first study in which ANO7 interacting proteins have been identified. Although further studies are needed, the findings reported here expand our understanding of the role and regulation of ANO7 in prostate cancer cells. Furthermore, these results are likely to introduce new targets for the novel cancer therapies.
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