A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Specific Lhc Proteins Are Bound to PSI or PSII Supercomplexes in the Diatom Thalassiosira pseudonana
Tekijät: Claudio Calvaruso, Anne Rokka, Eva-Mari Aro, Claudia Büchel
Kustantaja: AMER SOC PLANT BIOLOGISTS
Julkaisuvuosi: 2020
Lehti:Plant Physiology
Tietokannassa oleva lehden nimiPLANT PHYSIOLOGY
Lehden akronyymi: PLANT PHYSIOL
Vuosikerta: 183
Numero: 1
Aloitussivu: 67
Lopetussivu: 79
Sivujen määrä: 13
ISSN: 0032-0889
eISSN: 1532-2548
DOI: https://doi.org/10.1104/pp.20.00042
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/48458007
Despite the ecological relevance of diatoms, many aspects of their photosynthetic machinery remain poorly understood. Diatoms differ from the green lineage of oxygenic organisms by their photosynthetic pigments and light-harvesting complex (Lhc) proteins, the latter of which are also called fucoxanthin-chlorophyll proteins (FCP). These are composed of three groups of proteins: Lhcf as the main group, Lhcr that are PSI associated, and Lhcx that are involved in photoprotection. The FCP complexes are assembled in trimers and higher oligomers. Several studies have investigated the biochemical properties of purified FCP complexes, but limited knowledge is available about their interaction with the photosystem cores. In this study, isolation of stable supercomplexes from the centric diatom Thalassiosira pseudonana was achieved. To preserve in vivo structure, the separation of thylakoid complexes was performed by native PAGE and sucrose density centrifugation. Different subpopulations of PSI and PSII supercomplexes were isolated and their subunits identified. Analysis of Lhc antenna composition identified Lhc(s) specific for either PSI (Lhcr 1, 3, 4, 7, 10-14, and Lhcf10) or PSII (Lhcf 1-7, 11, and Lhcr2). Lhcx6_1 was reproducibly found in PSII supercomplexes, whereas its association with PSI was unclear. No evidence was found for the interaction between photosystems and higher oligomeric FCPs, comprising Lhcf8 as the main component. Although the subunit composition of the PSII supercomplexes in comparison with that of the trimeric FCP complexes indicated a close mutual association, the higher oligomeric pool is only weakly associated with the photosystems, albeit its abundance in the thylakoid membrane.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
